Services - Biologics - Antibody Technology
ELISA (Enzyme-Linked ImmunoSorbent Assay): The ELISA provides a powerful tool for the identification, detection, quantitation and characterization of either antibodies or their antigens. As the name would imply, all ELISAs employ enzyme-linked conjugates in the detection processes. ELISAs come in a variety of flavors: direct or indirect, antibody/antigen capture or sandwich, competitive or non-completive, color-based, fluorescent, chemiluminescent or radioactive. ELISAs are most often performed on 96-well microtiter plates for high throughput.
EIA (Enzyme Immunoassay): The terms EIA and ELISA are used interchangeably (see ELISA). Essentially, EIAs employ enzyme-linked antibodies for the identification, detection, quantitation and/or characterization of either antibodies or their antigens. EIAs are usually performed on 96-well microtiter plates for high throughput.
Immunoblot: The Immunoblot assay combines the high resolution of gel electrophoresis with the specificity and sensitivity of an immunoassay. While not as rapid as the ELISA assay, it does provide more information regarding the antigen. Immunoblotting may also be used to accurately characterize the antibody.
Protein Conjugation: Protein conjugation is the covalent linkage of a protein, such as an antibody, to some form of label. Conjugation provides a rapid means of detection for that protein or antibody. Proteins are commonly labeled with biotin, fluorochromes, enzymes or radiolabels. Conjugation is most often performed using glutaraldehyde, carbodiimide, succinimide esters, benzidine, periodate or isothiocyanate.
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